Which method is NOT typically used to test for VND?

Hemagglutination inhibition is not typically used as a method to test for Virulent Newcastle Disease (VND). The other methods listed are standard diagnostic techniques for VND.

Viral isolation via embryonate SPF (Specific Pathogen Free) eggs is widely used, as it allows for the propagation of the virus and subsequent analysis. Culture and PCR (Polymerase Chain Reaction) are critical for detecting viral RNA, as PCR is highly sensitive and can identify even small quantities of viral genetic material. Serological ELISA (Enzyme-Linked Immunosorbent Assay) testing plays a key role in detecting specific antibodies against the virus, helping to determine past or present infections.

In contrast, hemagglutination inhibition, while it can be used for certain viruses, is not a primary method for VND due to the specific nature of the virus and the need for more sensitive and specific detection methods.